T4 (10 test) for Insight V-IA Analyser (WD1562)



The InSight V-IA Thyroxine(T4)Rapid Quantitative Test is a fluorescence
immunoassay used with the InSight V-IA Veterinary Immunoassay Analyser for
quantitative determination of canine or feline T4 concentration in canine or feline
serum or plasma. The test is used to assist in the evaluation of thyroid function.

Test principper

This test employs a quantitative competitive fluorescence immunoassay technique.
A competitive binding assay is based upon the competition of labelled and
unlabelled analytes for a limited number of antibody binding sites. Unbound
antibodies and immunocomplexes migrate along the nitrocellulose membrane
towards the test line. The unbound antibodies are then captured by antigens
immobilised on the test line. The more T4 in the patient specimen, the more
immunocomplexes are formed, thus the less fluorescent-labelled antibodies
captured on the test line. The fluorescent signal intensity reflects the amount of T4
captured and is processed in InSight V-IA Veterinary Immunoassay Analyser. The
T4 concentration is expressed in nmol/L or µg/dl.

Opbevaring og stabilitet

1.Store the test kit at 4~30℃ up to the expiration date.
2.Once the pouch has been opened, the test should be performed within an hour.
3.If removed from the refrigerator, allow 30 minutes for the test to attain room temperature before testing.

Prøvesamling og forberedelse

The test can be performed with either serum or plasma.
1.Following standard phlebotomy venipuncture procedure, collect whole blood
sample using a blood collection tube. If a plasma sample will be used, use a blood
collection tube containing lithium heparin anticoagulant. If a serum sample will be
used, use a plain serum tube.
2.Separate serum or plasma from blood within 2 hours after blood collection. If a
sample appears to be severely haemolysed, another sample should be obtained and
3.The test should be performed immediately after the specimen collection. If the
test cannot be performed within 2 hours after blood collection, store the sample at
2℃~8℃ for no longer than 48 hours. For long-term storage, samples should be
kept below -20℃.
Bring all materials to room temperature before use. Frozen samples must be
completely thawed and mixed well prior to testing. Samples should not be
frozen and thawed repeatedly. Only clear, non-haemolysed specimens should
be used.

Test procedure

Refer to InSight V-IA Veterinary Immunoassay Analyser User Manual for complete
instructions for use of the analyser.
1.Set the test cartridge on a clean, level horizontal surface.
2.Make sure that the cartridge Lot No. matches with the ID Chip No. Insert the ID
Chip into the analyser. Be aware not to touch the insertion tip of the ID Chip. Press
“Read ID Card” on the test screen.
3.Pipette 75μl of prepared sample into the buffer tube, gently mix well. Vigorous
agitation and foaming should be avoided.
4.Pipette 75μl of mixed sample dilution to the sample well of the test cartridge.
Avoid forming bubbles.
5.Please refer to Section V in the InSight V-IA Veterinary Immunoassay Analyser
User Manual for details.
a) Quick Test Mode: Set the timer for 15 minutes, start the timer immediately after
adding the sample mixture to the sample well. Once the timer has counted down,
insert the test device immediately into the cartridge holder of the analyser and click
‘Test’. The instrument will scan the test device automatically and show the test
b) Standard Test Mode: Insert the test device into the cartridge holder of the
analyser immediately after adding the sample to the sample well, click ‘Test’. The
analyser will start to countdown and read the test results automatically.
6.Results are displayed on the main screen and printed automatically.

Fortolkning af testresultater

The InSight V-IA Veterinary Immunoassay Analyser calculates T4 test results
automatically and displays the concentration of T4 on the screen. For further
information, refer to the User Manual for the InSight V-IA Veterinary Immunoassay
Reference range of Thyroxine in canine or feline blood:
1.Detection range: 8~100 nmol/L / 0.62~7.77 µg/dL
Reference Ranges:

nmol/L ug/dL
Hund Lav
< 15
15 – 50
> 50
< 1,2
1,2 – 3,9
> 3,9
Kat Lav
< 15
15 – 60
> 60
< 1,2
1,2 – 4,7
> 4,7

Each Laboratory should establish a reference range that is representative of
the population to be evaluated.


Each InSight V-IA T4 Rapid Quantitative Test contains an internal control for
routine quality control requirements. This internal quality control is performed each
time a patient sample is tested. If an invalid result from the internal control occurs,
the analyser will display an error message, indicating that another test should be


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