Progesterone (10 test) for Insight V-IA TEST (WD1561)



The InSight V-IA canine Progesterone (cProg) Rapid Quantitative Test is a
fluorescence immunoassay used with the InSight V-IA Veterinary Immunoassay
Analyser for quantitative determination of progesterone concentration in canine
serum or plasma. The test is used as an aid to track ovulation, determine the best
time for breeding or detect early pregnancy failure.

Test principper

This test employs a quantitative competitive fluorescence immunoassay
technique. A competitive binding assay is based upon the competition of labelled
and unlabelled analytes for a limited number of antibody binding sites. Unbound
antibodies and immunocomplexes migrate along the nitrocellulose membrane
towards the test line. The unbound antibodies are then captured by antigens
immobilised on the test line. The more progesterone in the patient specimen, the
more immunocomplexes are formed, thus the less fluorescent-labelled antibodies
captured on the test line. The fluorescent signal intensity reflects the amount of
cProg captured and is processed in InSight V-IA Veterinary Immunoassay
Analyser. The cProg concentration is expressed in ng/mL or nmol/L.

Opbevaring og stabilitet

1.Store the test kit at 4~30℃ up to the expiration date.
2.Once the pouch is opened, the test should be performed within an hour.
3.If removed from the refrigerator, allow 30 minutes for the test to attain room
temperature before testing.

Prøvesamling og forberedelse

The test can be performed with either serum or plasma.
1.Following standard phlebotomy venipuncture procedure, collect whole blood
sample using a blood collection tube.
If a plasma sample will be used, use a blood collection tube containing lithium
heparin anticoagulant. If a serum sample will be used, use a plain serum tube.
2.Separate serum or plasma from blood within 2 hours after blood collection. If
a sample appears to be severely haemolysed, another sample should be obtained
and tested.
3.The test should be performed immediately after the specimen collection. If the
test cannot be performed within 2 hours after blood collection, store the sample
at 2℃~8℃ for no longer than 48 hours. For long-term storage, samples should
be kept below -20℃.
Bring all materials to room temperature before use. Frozen samples must be
completely thawed and mixed well prior to testing. Samples should not be
frozen and thawed repeatedly. Only clear, non-haemolysed specimens should
be used.

Test procedure

Refer to the InSight V-IA Veterinary Immunoassay Analyser User Manual for
complete instructions for use of the analyser.
1.Set the test cartridge on a clean, level horizontal surface.
2.Make sure that the test cartridge Lot No. matches with the ID Chip No. Insert
the ID Chip into the analyser. Be aware not to touch the insertion tip of the ID
Chip. Press ‘Read ID Card’ on the test screen.
3.Pipette 75μl of prepared sample into the buffer tube, gently mix well. Vigorous
agitation and foaming should be avoided.
4.Pipette 75μl of mixed sample dilution to the sample well of the test cartridge.
Avoid forming bubbles.
5.Please refer to Section V in the InSight V-IA Veterinary Immunoassay Analyser
User Manual for details.
a) Quick Test Mode: Set the timer for 15 minutes, start the timer immediately
after adding the sample mixture to the sample well. Once the timer has counted
down, insert the test device immediately into the cartridge holder of the analyser
and click ‘Test’. The analyser will scan the test device automatically and show
the test result.
b) Standard Test Mode: Insert the test device into the cartridge holder of the
analyser immediately after adding the sample to the sample well, click ‘Test’.
The analyser will start to countdown and read the test result automatically.
6.Results are displayed on the main screen and printed automatically.

Fortolkning af testresultater

The InSight V-IA Veterinary Immunoassay Analyser calculates progesterone test
results automatically and displays the concentration of progesterone on the screen.
For further information, refer to the User Manual for the InSight V-IA Veterinary
Immunoassay Analyser.
Reference range of Progesterone in canine blood:
1.Detection range: 1-50 ng/mL / 3.18-159 nmol/L
2.Reference Range:

ng/mL nmol/L
Not in heat or proestrus < 1,0 < 3,18
Pre LH Surge 1 – 5 3,18 – 15,9
Ovulation 5 – 10 15,9 – 31,8
The best breeding period (breed depent) 10 – 20 31,8 – 63,6
Diestrus > 30 > 95,4

Each Laboratory should establish a reference range that is representative of
the population to be evaluated. * 1ng/mL=3.18nmol/L


Each InSight V-IA cProg Rapid Quantitative Test contains an internal control for
routine quality control requirements. This internal quality control is performed
each time a patient sample is tested. If an invalid result from the internal control
occurs, the analyser will display an error message, indicating that another test
should be performed.


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