Cortisol (10 test) for Insight V-IA (WD1564)

Beskrivelse

Anvendelse

The InSight V-IA Cortisol Rapid Quantitative Test is a fluorescence immunoassay
used with the InSight V-IA Veterinary Immunoassay Analyser for quantitative
determination of canine or feline Cortisol concentration in canine or feline serum or
plasma. Cortisol is a hormone that regulates metabolism and is used to help diagnose
adrenal disorders.

Test principper

This test employs a quantitative competitive fluorescence immunoassay technique.
A competitive binding assay is based upon the competition of labelled and
unlabelled analytes for a limited number of antibody binding sites. Unbound
antibodies and immunocomplexes migrate along the nitrocellulose membrane
towards the test line. The unbound antibodies are then captured by antigens
immobilised on the test line. The more Cortisol in the patient specimen, the more
immunocomplexes are formed, thus the less fluorescent-labelled antibodies captured
on the test line. The fluorescent signal intensity reflects the amount of Cortisol
captured and is processed in InSight V-IA Veterinary Immunoassay Analyser. The
Cortisol concentration is expressed in nmol/L or µg/dL.

Opbevaring og stabilitet

1.Store the test kit at 4~30℃ up to the expiration date.
2.Once the pouch has been opened, the test should be performed within an hour.
3.If removed from the refrigerator, allow 30 minutes for the test to attain room
temperature before testing

Prøvesamling og forberedelse

The test can be performed with either serum or plasma.
1.Following standard phlebotomy venipuncture procedure, collect whole blood
sample using a blood collection tube.
If a plasma sample will be used, use a blood collection tube containing lithium
heparin anticoagulant. If serum sample will be used, use a plain serum tube.
2.Separate serum or plasma from blood within 2 hours after blood collection. If a
sample appears to be severely haemolysed, another sample should be obtained and
tested.
3.The test should be performed immediately after the specimen collection. If the test
cannot be performed within 2 hours after blood collection, store the sample at 2℃~
8℃ for no longer than 48 hours. For long-term storage, samples should be kept
below -20℃.
Bring all materials to room temperature before use. Frozen samples must be
completely thawed and mixed well prior to testing. Samples should not be
frozen and thawed repeatedly. Only clear, non-haemolysed specimens should
be used.

Test procedure

Refer to the InSight V-IA Veterinary Immunoassay Analyser User Manual for
complete instructions for use of the analyser.
1.Set the test cartridge on a clean, level horizontal surface.
2.Make sure that the test cartridge Lot No. matches with the ID Chip No. Insert the
ID Chip into the analyser. Be aware not to touch the insertion tip of the ID Chip.
Press ‘Read ID Card’ on the test screen.
3.Pipette 75μl of prepared sample into the buffer tube and gently mix well. Vigorous
agitation and foaming should be avoided.
4.Pipette 75μl of mixed sample dilution to the sample well of the test cartridge.
Avoid forming bubbles.
5.Please refer to Section V in the InSight V-IA Veterinary Immunoassay Analyser
User Manual for details.
a) Quick Test Mode: Set the timer for 15 minutes, start the timer immediately after
adding the sample mixture to the sample well. Once the timer has counted down,
insert the test cartridge immediately into the cartridge holder of the analyser and
click ‘Test’. The analyser will scan the test device automatically and show the test
result.
b) Standard Test Mode: Insert the test device into the cartridge holder of the
analyser immediately after adding the sample to the sample well and click ‘Test’.
The analyser will start to countdown and read the test results automatically.
6.Results are displayed on the main screen and printed automatically.

Fortolkning af testresultater

The InSight V-IA Veterinary Immunoassay Analyser calculates Cortisol test results
automatically and displays the concentration of Cortisol on the screen. For further
information, refer to the User Manual for the InSight V-IA Veterinary Immunoassay
Analyser.
Reference ranges of Cortisol in canine or feline blood:

1.Detection Range: 10~1000 nmol/L / 0.36~36.18 µg/dL

2.ACTH Stimulation Test:

Før ACTH Efter ACTH Cortisol værdi
< 55 nmol/L (<1,99 ug/dL) < 55 nmol/L (<1,99 ug/dL)

55-166 nmol/L (1,99 – 6,00 ug/dL)

Addisons sygdom

Ingen konklusion

< 55-166 nmol/L (<1,99 – 6,00 ug/dL) 55-166 nmol/L (1,99 – 6,00 ug/dL)

166 – 500 nmol/L (6,00 – 18,09 – 23,99 ug/dL)

500 – 663 nmol/L (18,09 – 23,99 ug/dL)

> 663 nmol/L (>23,99 ug/dL)

Ingen konklusion

Normal

Muligvis Crushing syndrom

Crushing syndrom

 

3.LDDST

Cortisol værdi efter 4 timer Cortisol værdi efter 8 timer
Normal < 39 nmol/L (1,4 ug/L) < 39 nmol/L (1,4 ug/L) Normal
Normal eller forhøjet > 39 nmol/L eller > 50% basisværdi > 39 nmol/L eller > 50% basisværdi Crushing syndrom
Normal eller forhøjet < 39 nmol/L eller < 50% basisværdi > 39 nmol/L eller > 50% basisværdi PDH
Normal eller forhøjet > 39 nmol/L eller > 50% basisværdi > 39 nmol/L eller < 50% basisværdi PDH
Normal eller forhøjet < 39 nmol/L eller < 50% basisværdi > 39 nmol/L eller < 50% basisværdi PDH

 

4. HDDST

Cortisol basisværdi Cortisol værdi efter 4 timer Cortisol værdi efter 8 timer
Normal eller forhøjet < 39 nmol/L eller < 50% basisværdi > 39 nmol/L eller > 50% basisværdi PDH
Normal eller forhøjet > 39 nmol/L eller > 50% basisværdi < 39 nmol/L eller < 50% basisværdi PDH
Normal eller forhøjet < 39 nmol/L eller < 50% basisværdi < 39 nmol/L eller < 50% basisværdi PDH
Normal eller forhøjet < 39 nmol/L eller > 50% basisværdi > 39 nmol/L eller > 50% basisværdi Der er behov for flere tests for at skelne PDH og binyreafhængige sygdomme.

Each Laboratory should establish a reference range that is representative of the population to be evaluated.

Kvalitetskontrol

Each InSight V-IA Cortisol Rapid Quantitative Test contains an internal control for
routine quality control requirements. This internal quality control is performed each
time a patient sample is tested. If an invalid result from the internal control occurs,
the analyser will display an error message, indicating that another test should be
performed

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