cCRP (10 tests) for Insight V-IA

Application

InSight V-IA C-Reactive Protein (cCRP) Rapid Quantitative Test is a fluorescence immunoassay used for InSight V-IA Veterinary Immunoassay Analyzer for quantitative determination of serum or plasma cCRP concentration. The test can be used as a general marker of inflammatory response.

Test principles

1. This test employs a quantitative double antibody sandwich fluorescence
technique immunoassay.
2. The fluorescent signal intensity reflects the amount of cCRP captured and is
processed in the InSight V-IA Veterinary Immunoassay Analyzer. The cCRP
concentration is expressed in mg / L.

1. The test uses a quantitative dual antibody fluorescence immunoassay technique.
2. The intensity of the light emitted by the fluorescent substance reflects the amount of cCRP. This is measured in InSight V-IA Veterinary Immunoassay Analyzer. cCRP concentration is expressed in μg / L.

Storage and stability

1. The test kit is stored at 4-30 ℃ until the expiration date.
2. Once the bag is opened, the test must be performed within 1 hour.
3. If the test is stored in a refrigerator, 30 minutes must elapse before use so that the test can reach room temperature.

Sample collection and preparation

The test can be performed with either serum or plasma.

After venipuncture (phlebotomy), a whole blood sample is collected in a blood collection tube. If a plasma sample is used, use a blood collection tube containing lithium heparin anticoagulant. If a serum sample is used, use a serum separation tube.

2. Separate serum or plasma from the blood within 2 hours after taking the blood sample. If a sample is highly hemolyzed, a new sample is recommended.

3. The test must be performed immediately after sampling. If the test cannot be performed within 2 hours after blood collection, the test should be stored at 2 to 8 ℃, and for a maximum of 48 hours. If the samples are to be stored for a longer period of time, they must be stored at a temperature below -20 ℃.

When a test is to be used after long-term storage and / or freezing, it must be thawed to room temperature and then mixed well before use. Note that the sample must not be frozen again after thawing.

Test procedure

See the user guide for InSight V-IA Veterinary Immunoassay Analyzer for complete guidance to the analyzer.

1. Place the test cartridge on a clean, level surface.
2. Make sure that the batch number of the test cartridge matches the ID chip number, and then insert the ID Chip into the analyzer. Be careful not to touch the tip of the ID Chip. Tap 'Read ID card' on the test screen.

3. Use a 10 μl pipette to transfer the sample to the buffer tube and mix gently. Vigorous stirring and foaming should be avoided.

4. Pipette 75 μl of mixed sample dilution into the sample well of the test cartridge. Avoid the formation of bubbles in the sample.

5. See section V i InSight V-IA Veterinary Immunoassay Analyzer User Manual for details.
   
   a) Quick Test Mode: Set the timer to 3 minutes and start the timer immediately after adding the sample mixture to the sample well. When the timer is complete, insert the test cartridge into the cartridge holder on the analyzer. Then click on 'Test'. The analyzer automatically scans the test unit and then displays the test result.

   b) Standard Test Mode: Insert the test unit into the cartridge holder on the analyzer immediately after adding the sample to the sample well, then click 'Test'. The analyzer starts counting down and automatically reads the test result.

6. The result is displayed on the main screen and printed automatically.

Interpretation of test results

InSight The V-IA Veterinary Immunoassay Analyzer automatically calculates the cCRP test result and displays the concentration of cCRP on the screen.
For more information, see the user guide for InSight V-IA Veterinary Immunoassay Analyzer.

Reference area: <10 mg / L

NOTE: Each clinic should create a reference area that is representative of the animals being tested at the clinic.

Quality control

Every InSight The V-IA cCRP Rapid Quantitative Test includes a quality control that is automatically performed each time a sample is tested.

If the quality control detects an invalid result, the analyzer will display an error message and the sample must be retaken.